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IDT launches new approach to CRISPR with proven increase in genome-editing potency
January 2016
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Integrated DNA Technologies
 
Integrated DNA Technologies introduces a new approach to CRISPR—its innovative Alt-R CRISPR-Cas9 System, based on the naturally occurring S. pyogenes CRISPR RNA system. The Alt-R CRISPR-Cas9 System greatly improves genome-editing potency thanks to research-optimized crRNAs (CRISPR RNA) and tracrRNAs (trans-activating crRNA). Along with improved potency and safety, the system saves time by providing easy, ready-to-use RNA reagents that also reduce cell toxicity by avoiding activation of cellular innate immune responses.
 
The CRISPR/Cas9 system has become a leading tool for modifying genomes of organisms from E. coli to humans. Chimeric single-guide RNAs are commonly cloned into plasmid vectors, and then used to express the RNA trigger in cells. However, the use of plasmid vectors to drive genome editing lacks efficacy and is suspected of causing significant off-target effects, while sgRNAs obtained by in-vitro transcription can be expensive and cytotoxic through activation of the innate immune system in many mammalian cell types. Manufacture of high-quality sgRNAs by chemical synthesis is also difficult and costly due to their length.
 
Integrated DNA Technologies

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