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CRISPR gRNA lentivector cloning kits
September 2016
SHARING OPTIONS:

AMSBIO
 
The new kits enable rapid, precisely directional cloning of your DNA duplex encoded gRNA structure at efficiencies of greater than 95 percent. Also incorporating a pre-linearized lentivector, the kit eliminates the need for tedious preparation of a lentivector backbone. The lentivector included in each AMSBIO kit encodes an antibiotic marker or a dual marker (a fluorescent-antibiotic fusion marker) allowing generation of stable cell lines for long-term gRNA expression. There are two promoters available: U6 and an optional inducible H1 promoter. The gRNA lentivectors driven by the inducible H1 promoter can be used for constitutive high expression without the need for induction. This modified H1 promoter allows the inducible expression of gRNA when the tetracycline repressor protein is present in advance and the U6 promoter is the constitutive promoter.
 
AMSBIO

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