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A new field option for Ebola diagnosis
September 2015
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CORALVILLE, Iowa—Scientists from Integrated DNA Technologies (IDT) have joined with a research team from Battelle to design and validate an RT-qPCR assay capable of detecting Ebola in the field. The IDT PrimeTime Ebola qPCR Assay detects Zaire ebola virus, the species responsible for the recent outbreak in West Africa, and has a PCR efficiency of 98.9 percent. In addition, the use of ZEN Double-Quenched Probes from IDT reduces assay background and increases signal compared to traditional single-quenched probes. Dr. Paul Pickering of Ubiquitome Limited is leading the drive to develop the assay for use with the Ubiquitome Freedom4 device, a portable PCR machine that processes four reactions in roughly an hour and is controlled via an iPhone or laptop. The Freedom4 and the PrimeTime Ebola qPCR Assay are being submitted to the U.S. Food and Drug Administration for emergency use authorization.

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