Back To: Home : Featured Technology : PCR/qPCR

CLICK HERE FOR WHAT'S NEW IN:
 

Nano gene therapy
01-22-2007
by Randall C Willis  |  Email the author
SHARING OPTIONS:

CLEVELAND—Because many ocular diseases have genetic origins, the eye has become a popular target for gene therapy. Concerns about viral vectors, however, led researchers at Copernicus Therapeutics and the University of Oklahoma Health Sciences Center to explore DNA delivery using nanoparticles.
 
As they reported in PLoS One, the researchers tagged DNA expressing enhanced green fluorescent protein (EGFP) to two nanoparticle formulations and injected each of them into two eye compartments. Using quantitative RT-PCR and fluorescence microscopy to detect EGFP expression, the researchers noted good expression of the transgene compared to the injection of "naked" DNA.
 
They also noted a strong correlation between injection site—intravitreal versus subretinal—and tissue-specific EGFP expression, offering the possibility of precisely targeted gene therapy. Furthermore, using electroretinography to test retinal activity, the researchers determined that the nanoparticles had no toxic impact on eye tissues.

Back


PAGE UTILITIES


CONTACT US
DDNEWS
Published by Old River Publications LLC
19035 Old Detroit Road
Rocky River, OH USA 44116
Ph: 440-331-6600  |  Fax: 440-331-7563
 
© Copyright 2017 Old River Publications LLC. All righs reserved.  |  Web site managed and designed by OffWhite.