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▀-arrestin assays with 30-second detection
June 2007
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With the PathHunter Flash Detection Kit, from DiscoveRx, ▀-arrestin recruitment by a GPCR can now be detected in 30 secondsŚpermitting screening of up to 1,000,000 compounds in 48 hours. Fast PathHunter ▀-arrestin chemiluminescent assays, unlike lengthy reporter gene assays, greatly minimize the opportunity for off-target effects. A ligand-activated, GPCR-arrestin interaction combines two ▀-galactosidase fragments, enabling rapid detection in a homogeneous format. The kit is designed for plate readers with onboard fluidics and flash detection mode. Additionally, screening campaigns can be accelerated by conducting ▀-arrestin and calcium assays in the same well when using instruments capable of real-time fluorescence and flash chemiluminescence.
 
DiscoveRx Corp.
510-979-1415

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