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ReaX assays speed up bacterial identification
December 2007
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Q Chip, a developer of microencapsulation solutions, has developed a new PCR tool which speeds up bacterial identification, with greater accuracy and less variability. ReaX Assay 16S beads contain all the reagents required to perform 16S rRNA gene amplification, including a high-performance Taq polymerase, dNTPs and the universal 16S rRNA gene-specific primers 27F and 907R. Only template DNA is required prior to running the PCR protocol. Amplification is confirmed using end point PCR. Identification using the ReaX Assay 16S beads is based on PCR amplification of the 16S ribosomal RNA gene. In a comparative study of the identification of E. coli and S. aureus, the ReaX Assay 16S PCR beads generated identical PCR bands for each triplicate and for each organism, compared with conventional liquid-based PCR methods. However, with the ReaX Assay 16S PCR beads less manual pipetting was required, with fewer opportunities for contamination, and reduced pipetting error was also observed, leading to less variability between reactions. PCR reaction set-up using the ReaX Assay 16S beads required no prior optimization and was quicker and more user-friendly to perform compared to conventional liquid-based PCR set-up.
Q Chip Life Science
+44 (0)29 2068 2092

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