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Master Mixes for Use on Thermal Block-Based PCR Instruments, Roche Applied Science
August 2005
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The FastStart TaqMan Probe Master and the FastStart SYBR Green Master from Roche Applied Science are new ready-to-use reagent mixes that simplify the preparation of samples for quantitative PCR (qPCR) and two-step quantitative reverse transcription PCR (qRT-PCR). These master mixes are optimized for use with most real-time PCR instruments other than the LightCycler 2.0 System Instruments. They are available in two formulations, one that contains the ROX reference dye and one without ROX. In combination with a real-time PCR instrument, suitable PCR primers, and a hydrolysis probe, FastStart TaqMan Probe Master allows very sensitive detection and quantification of defined DNA sequences. The FastStart TaqMan Probe Master shows excellent performance in combination with probes in the Roche Universal ProbeLibrary.
 
Roche Applied Science 
800-262-4911

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