Back To: Home : Featured Technology : PCR/qPCR

CLICK HERE FOR WHAT'S NEW IN:
 

Sample preparation reagent provides reverse transcription-ready RNA in five minutes
December 2009
SHARING OPTIONS:

Bio-Rad Laboratories Inc.

Bio-Rad Laboratories Inc. has launched the iScript RT-qPCR Sample Preparation Reagent for rapid isolation of total RNA and enabling reverse transcription and real-time PCR to be performed directly from cell lysates. iScript employs selective lysis of the cell membrane while leaving the nuclear membrane intact to achieve DNA-free RNA within five to 10 minutes. The single buffer formulation contains all the components needed to perform cell lysis, RNA stabilization and genomic DNA removal for sensitive, quantitative gene expression analysis, and it offers sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates. It also enables multiplex real-time detection of up to four targets from as few as 10 cells. Upon exposure of cells to iScript, cell disruption and lysis occurs in approximately 30 seconds with the aid of mild vortexing. The reagent also stabilizes RNA and effectively inhibits intracellular ribonucleases. A two-minute, high-speed centrifugation yields total RNA that can be collected in the supernatant while the nuclei containing genomic DNA form a pellet and can be discarded. RNA isolated with iScript is stable for up to six months when stored at -20C.  

Bio-Rad Laboratories Inc.
www.bio‑rad.com/reversetranscription
(800) 424-6723 

Back


PAGE UTILITIES


CONTACT US
DDNEWS
Published by Old River Publications LLC
19035 Old Detroit Road
Rocky River, OH USA 44116
Ph: 440-331-6600  |  Fax: 440-331-7563
 
© Copyright 2017 Old River Publications LLC. All righs reserved.  |  Web site managed and designed by OffWhite.