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Genome Amplification Kits, Sigma-Aldrich
November 2005
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Sigma-Aldrich announced two additions to the GenomePlex Whole Genome Amplification (WGA) family of products: the GenomePlex Complete Whole Genome Amplification Kit and the GenomePlex WGA Reamplification Kit. Researchers performing whole genome amplification assays who have a need for increased amplification accuracy in addition to finding a method that preserves precious source material may find those options satisfied with the GenomePlex Complete Whole Genome Amplification Kit and the GenomePlex WGA Reamplification Kit. The GenomePlex Complete WGA Kit contains the same reagents as the original GenomePlex WGA Kit (WGA1), as well as an optimized enzyme, WGA DNA Polymerase. Sigma claims this enzyme provides a high degree of accuracy in amplification and no amplicon in negative control reactions. Assays using the GenomePlex Complete WGA Kit provide results showing complete representation of the entire genome with no detectable allele bias. The GenomePlex WGA Reamplification Kit allows subsequent reamplifications of the initial whole genome amplification product. The successive reamplification process provides microgram quantities of DNA with little genetic bias when compared to the original genome. The GenomePlex WGA Reamplification kit contains the WGA DNA Polymerase and 10X Master Amplification Mix. The optimized enzyme, WGA DNA Polymerase, decreases the background in the reactions and leads to more accurate results in downstream PCR or hybridization techniques.
 
Sigma-Aldrich
800.325.5832

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