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Universal Cloning Following PCR, Clonetech
January 2006
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Clonetech's new In-Fusion PCR Cloning Kits allow users to fuse a PCR-amplified insert into any vector, linearized at any restriction site, in a simple 30 minute reaction. The In-Fusion PCR Cloning Kit is designed for directional cloning of PCR products without the need for restriction digestion of the insert, ligation, or end polishing. In-Fusion kits are available in different formats in order to accomodate various user applications such as different vector systems, reaction numbers, or automation options. A lyophilized kit format is offered for ease-of-use and consistency, while a liquid format provides maximum versatility. Both formats have been extensively tested and provide equivalent cloning efficiency for small-scale and high-throughput experiments. Vectors of up to 12 kb and inserts of up to 8 kb have been used successfully with both formats.
 
Clonetech
650.919.7382

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