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96-well PPAR Transcription Factor Assay Kit, Cayman Chemical Co.
December 2005
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The Cayman Chemical PPAR Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPAR binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPAR is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide sensitive colorimetric readout at 450 nm.
 
Cayman Chemical
800.364.9887

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