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GeneMaker Gene Synthesis, Blue Heron Bio
GeneMaker Gene Synthesis is available for less than the cost of PCR cloning. According to Blue Heron Bio, their novel, non-PCR GeneMaker gene synthesis provides perfectly accurate clones rapidly and economically.
GeneMaker's proprietary software designs the build strategy and stores the instructions in a database. Double-stranded oligonucleotides ("duplexes") are made to the specifications designated by the design software by GeneMaker's proprietary gene assembly instrument. The gene assembly instrument sequentially adds "duplexes" to a solid matrix in a proprietary column.
Blue Heron claims three advantages of gene synthesis over PCR: 1) this process can be used to assemble any length DNA fragment, from 50 base pairs to 40,000 base pairs or longer; 2) the sequential addition avoids errors of order that commonly occur during PCR synthesis, and 3) the automation enables high throughput, highly accurate gene production quickly and at a low cost.
Blue Heron Bio 888-368-5923