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Human Splice Variant Discovery, Stratagene Corp.
May 2006
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Stratagene Corp. announced the commercial availability of the Human SpliceMiner cDNA Library Panel. This new product greatly simplifies the process of discovering rare and unique alternative splice forms that would have otherwise been difficult or impossible to detect by other methods. Many of the Stratagene SpliceMiner panel cancer cell lines are in use at the National Cancer Institute of the US National Institutes of Health to screen for anticancer drugs. The Human SpliceMiner cDNA library panel consists of ~4.6 million primary cDNA clones derived from 15 high-quality cDNA plasmid libraries representing 28 cancer cell lines and 32 normal human tissues, including 14 sub-regions of the brain. The SpliceMiner panel design facilitates capture of rare mRNAs and is enriched for complete cDNA transcripts, while maintaining mRNA representation found in the original human source material.  Unlike a traditional cDNA library where the entire content is pooled into one highly complex sample, SpliceMiner cDNA clones are arrayed into 384 lower-complexity pools (4 x 96-well format). The pools are strategically segregated to enhance the probability of amplifying one specific splice variant per well. The segregated pool in each well contains ~12,000 clones. By amplifying multiple lower-complexity pools, the statistical probability of finding a single splice variant of a particular mRNA is vastly improved, especially for low-to-medium abundance transcripts.
Stratagene
www.stratagene.com
800.894.1304 

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