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Multiplex assay for analysis of heat shock proteins
August 2009
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Assay Designs Inc.
 

Assay Designs Inc. has announced the release of the first commercially available multiplex assay dedicated to the analysis of heat shock proteins and molecular chaperones, the MultiBead HSP/Chaperone 8-Plex Kit. The bead-based multiplex immunoassay enables measurement of HSP client proteins (Akt and Akt phosphor-Ser473) and HSPs (Hsp27 phospho Ser15, Hsp27 phospho-Ser82, Hsp40, Hsp60, Hsp70 and Hsp90 alpha) in cell lysates. The assay utilizes monoclonal antibodies or antigen affinity purified polyclonal antibodies covalently coupled to latex beads. The detection antibodies are conjugated to biotin followed by a streptavidin-PE conjugate and analyzed on a dual-laser flow cytometer. The HSP/Chaperone panel provides a sensitive, rapid and specific method for accurately determining analyte levels in cell lysates.

Assay Designs Inc.

800-833-8651

www.assaydesigns.com


 

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