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Organic dye for violet lasers
October 2010
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Beckman Coulter Inc.  
 
Beckman Coulter Inc. introduces Krome Orange, a novel violet-excited organic dye that expands the range of available choices and dye sensitivity limits for violet lasers.  Common gating markers can be easily transferred to this fluorochrome, freeing other channels for use with more esoteric markers and enabling more versatile 10-color flow cytometry applications. Krome Orange has excitation and emission maxima of 398 nm and 528 nm respectively, and provides a brighter signal than Pacific Orange conjugates with equivalent spectral overlaps into adjacent channels. Krome Orange conjugates show optimal performance with violet laser excitation and a 550/40 bandpass filter (the standard FL10 channel on flow cytometry systems), while no excitation is detected using a 488 nm laser.   
 
Beckman Coulter Inc.
(800) 742-2345
www.beckmancoulter.com
 

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