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HCP antibody evaluation workflow
March 2013
SHARING OPTIONS:
Bio-Rad
Bio-Rad introduces a robust workflow to more effectively
evaluate antibodies that measure host cell protein impurities in
biotherapeutics. The workflow is based on 2D electrophoresis and Western
blotting, and takes two days to complete. Proteins are separated based on
isoelectric point in IPG strips using the PROTEAN il2 IEF system, then
separated based on molecular weight by SDS-PAGE on TGX precast gels, which are
then processed with the Trans-Blot Turbo system. Fluorescent protein staining
is performed, followed by Western blotting and then imaging.
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