Bio-Rad introduces a robust workflow to more effectively evaluate antibodies that measure host cell protein impurities in biotherapeutics. The workflow is based on 2D electrophoresis and Western blotting, and takes two days to complete. Proteins are separated based on isoelectric point in IPG strips using the PROTEAN il2 IEF system, then separated based on molecular weight by SDS-PAGE on TGX precast gels, which are then processed with the Trans-Blot Turbo system. Fluorescent protein staining is performed, followed by Western blotting and then imaging.

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