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Assay kits for measuring complete range of HDAC enzymes
March 2010
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AMSBIO
 
 
AMSBIO has announced two new histone deacetylase (HDAC) cell-based assay kits that provide a tool for studying the activity and inhibition of the full range of HDAC enzymes (1 to 11). The kits provide a fast and fluorescence-based method that eliminates radioactivity, extractions or chromatography, as are often used in traditional HDAC assays. By using a cell-permeable HDAC substrate in the new assay kits, the activity of various protein lysine-specific deacetylases, including HDAC-containing complexes, can be measured in intact cells in a simple and homogenous manner. The fluorescence of the deacetylated reaction product can be analyzed using a plate reader or a fluorometer. The new assay procedures require only two easy steps, each performed on a single microtitre plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity. Second, deacetylation of the substrate sensitises the substrate, so that further treatment with the lysine developer produces a fluorophore. Both the HDAC and HDAC class2a assay are well suited for high-throughput screening applications. HDAC inhibitors and antibodies, together with all 11 active HDAC enzymes, are available. Active Sirtuin proteins are also available separately.  
 
AMSBIO
 
+44- 1235-828200
 
 

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