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Sub-2-micron UHPLC column for reversed-phase protein characterization
November 2011
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Agilent Technologies Inc.
 
Agilent Technologies Inc. introduces its ZORBAX Rapid Resolution High Definition 300SB-C8, 1.8 Ám columns for liquid chromatography. The 1.8 Ám particles expand Agilent's range of wide-pore, 300┼ columns for better recovery, resolution and scalability in protein analysis. These RRHD columns are used in ultra-high- performance liquid chromatography (UHPLC) to analyze the primary structure of proteins, confirm protein identity, quantify post-translational modifications and provide impurity profiles for biotherapeutic protein discovery and production. The 1.8 Ám particle size and 300┼ pore size brings UHPLC efficiency, resolution and robust quantitation to reversed-phase protein separations. Additionally, the columns are stable up to 1,200 bar. Agilent's proven StableBond technology delivers symmetrical peaks even at low pH when using trifluoroacetic or formic acid mobile-phase modifiers, without compromising column life. Baselines are steady with UV and MS detection. The new columns are available in lengths of 50 mm and 100 mm to meet the needs of analysts for speed or resolution.  
 
Agilent Technologies Inc.
(877) 424-4536
www.agilent.com   
 

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